山东发现草地贪夜蛾为害马铃薯

重大迁飞性害虫草地贪夜蛾Spodoptera frugiperda(J.E.Smith)侵入我国后,截至2019年9月17日,已经在全国25个省份查见。2019年9月,山东省滕州市姜屯镇秋马铃薯地块发现疑似该虫为害,经鉴定,确认为草地贪夜蛾,对被害地块情况、马铃薯被害状、被害率进行了调查及描述,为该虫在马铃薯上的防治提供参考。     

Salvianolate attenuates oxidative stress injury of human endothelial EA.hy926 cells induced by hydrogen peroxide

AIM: To investigate the effect of salvianolate on oxidative damage induced by hydrogen peroxide in human endothelial EA.hy926 cells.METHODS: EA.hy926 cells were cultured in vitro and divided into the following groups:control group, damage group, and anti-damage groups (salvianolate+damage groups). The cell viability was measured by CCK-8 assay. The migration ability of the EA.hy926 cells was detected by Transwell assay. The content of nitric oxide (NO) in the culture supernatant of the EA.hy926 cells was examined. The levels of vascular endothelial growth factor (VEGF) were detected by ELISA. The apoptosis,mitochondrial membrane potential and intracellular superoxide anion content of the EA.hy926 cells were analyzed by flow cytometry. The protein levels of caspase-3, cleaved caspase-3, Bcl-2, Bax, NF-κB and p53 were determined by Western blot. RESULTS: Compared with damage group, the viability of EA.hy926 cells pretreated with salvianolate at different concentrations was significantly increased (P<0.05). The apoptotic rate was significantly decreased (P<0.05). Savianolate enhanced the migration ability of the cells. The levels of VEGF, NO and mitochondrial transmembrane potential were increased (P<0.05), and the intracellular ROS level was significantly decreased (P<0.05). The protein levels of NF-κB, p53, Bax and cleaved caspase-3 were significantly decreased, and the protein level of Bcl-2 was markedly increased(P<0.05). CONCLUSION: Savianolate reduces the damage of EA.hy926 cells by hydrogen peroxide exposure, and its mechanism may be related to the blocking of NF-κB signaling pathway.     

樱桃番茄抗青枯病砧木新品种海茄砧1 号的选育

海茄砧1 号是以野生茄子自交系HZ09-5 为母本，以从越南引进的茄子品种自交系HZ10-2 为父本育成的高抗青枯 病樱桃番茄砧木品种。植株生长势较强，第1 雌花节位约为第7 节，花为紫色，果实卵圆形，青熟果淡绿紫色或淡绿色，果 萼绿色，果长7～9 cm，果宽4～6 cm，单果质量为50～60 g，种子千粒重为3.2 g 左右，种皮浅黄色，高抗青枯病。海茄砧 1 号与樱桃番茄的嫁接亲和性好，共生性强，嫁接苗成活率高。嫁接后的樱桃番茄果萼开展，果面有光泽，可明显改善果实 VC、可溶性固形物和可滴定酸含量等品质，提高产量。适宜华南地区樱桃番茄的嫁接栽培。     

A rapid,simple, and sensitive immunoagglutination assay with silica nanoparticles for serotype identification of Pseudomonas aeruginosa

An agglutination test based on colored silica nanoparticles (colored SiNps) was established to detect serotypes of Pseudomonas aeruginosa. Monodisperse colored SiNps were used as agglutination test carriers. The colored SiNps were prepared through reverse microemulsion with reactive dyes, sensitized with 11 kinds of mono-specific antibodies against P. aeruginosa, and denoted as IgG-colored SiNps. Eleven kinds of IgG-colored SiNps were individually mixed with P. aeruginosa on a glass slide. Different serotypes of P. aeruginosa could be identified by agglutination test with evident agglutination. The P. aeruginosa could be detected in a range from 3.6 × 10⁵ to 3.6 × 10¹² cfu mL^?1. This new agglutination test was confirmed to be a specific, sensitive, fast, easy-to-perform, and cost-efficient tool for the routine diagnosis of P. aeruginosa.     

Impact of soil water content on maize responses to the plant growth-promoting rhizobacterium Azospirillum lipoferum CRT1

Members of the bacterial genus Azospirillum are root-associated bacteria that increase yield in cereals by promoting growth and alleviating drought stress. How plants integrate the many bacterium-derived growth-promoting stimuli with other environmental factors to generate a coordinated response remains unresolved. Using a commercial Azospirillum strain, A. lipoferum CRT1 and two host maize cultivars, it was observed that bacterization reduced the drought-induced increase in lateral root growth and enhanced the flood-induced increase in lateral root growth in the more drought- and flood-sensitive cultivar. In the other one, A. lipoferum CRT1 only elicited a moderate root growth response under low soil water potential. The photosynthetic potential and activity were increased in the earlier cultivar and decreased in the later one, irrespective of the soil water content. No impact of the bacterium was seen on the growth of the leaves of both cultivars under both stresses until the third leaf stage, therefore suggesting that it is a consequence of multiple primary adaptations to biotic and abiotic stresses. It is suggested that host–bacteria recognition leads to a stress-specific modulation of the root response and a differential stress-independent effect on photosynthesis. This is the first report of the impact of Azospirillum under flood conditions.     

鸡腿菇多糖减轻运动疲劳的机理分析

对鸡腿菇多糖的抗氧化活性进行了试验,试验表明,鸡腿菇多糖对·OH和DPPH·都具有一定的清除活性,且清除率与多糖的浓度成正比。这说明鸡腿菇多糖能够防止人体细胞膜过氧化损伤,减轻运动员的疲劳。     

基于数据包络分析模型下的食用菌产业发展策略

为了科学评价食用菌产业发展策略的有效性,建立了食用菌产业发展策略DEA效率评价指标体系,以食用菌产业发展策略中的一系列投入型策略作为输入指标,以食用菌企业的规模、消费者满意度、科技创新能力和市场竞争力等各种产出效益和效果作为输出指标,采用DEA评价方法对食用菌产业的发展策略的效率进行模拟评价。评价结果显示,对食用菌发展策略产生影响的各种因素是有效的,结果也为为食用菌发展策略的资源投入方向和数量提供了参考。     

共享经济模式下重庆食用菌旅游资源发展趋势及创新路径

从共享旅游文化、共享食用菌历史文化的角度,讨论了发展食用菌个性化旅游和体验式旅游的思路;结合食用菌绿色、健康和生态的核心体验元素,给出了依托食用菌绿色、生态的品牌优势,打造食用菌旅游产业的具体方法。在此基础上,探讨了重庆食用菌旅游资源的共享经济模式,提出以重庆食用菌美食为龙头,利用多种共享经济新业态来带动重庆食用菌旅游产业的发展。     

Typical Travel Events and the Reversal of Modern Image of China

In order to open up the Chinese market,Britain sent two missions to China in 1791 and 1816.The most powerful colonial empire in the world met the most powerful feudal empire in the world.The two sides led the negotiations and exchanges with the concepts of "tribute" and "equal diplomacy",and the failure of the missions of Macartney and Amherst to China was inevitable.The travel texts completed by the members of the mission recorded the process of travel to China and their views on China in detail.Two typical travel events in China completely reversed the western image of China.     

串番茄新品种红星302 的选育

红星302 是以抗病、耐低温弱光材料01-61 为母本，以01-23 为父本育成的串番茄一代杂种，无限生长类型，果实串收，果色亮红，果实椭圆形，酸甜适口，品质佳，VC 含量为364 mg · kg^-1，可滴定酸0.44%，可溶性糖4.25%，平均单果质量30.79 g，硬度为11.40×10⁵ Pa。单株结果数约为53个，产量4 900 kg ·（ 667 m²）^-1 左右，高抗蕨叶病毒病、青枯病，适宜河北地区冬季日光温室和早春保护地栽培。